Study of Decolorization Ability of Orange Azo β-NapHtol by Halomonaz Strain Gb bacteria and Optimization the Effects of Concentration, pH and Temperature

Document Type : Research Paper

Authors

1 Department of Chemical Engineering, Engineering College, Science & Research unit of Islamic Azad University,

2 Department of Chemical Engineering, Engineering College, Central Unit of Islamic Azad University, Tehran

3 Chemistry Department, Science College, North Unit of Islamic Azad University, Tehran

Abstract

Through this research, the ability of orange Azo β-NapHtol’s decolorization by Halomonaz Strain Gb bacteria and the efect of conectration, PH adn temoeratureon decolorization studied. The optimal conditions for decolorization were obtained via changing the level of different factors while keeping one factor in each test constant, is assayed. The final result  shows the maximum percentage of decolorization (88%) by 40 ppm concentration, 38˚C and pH = 9 after 6 days of process. As a result of testing in a  wide range of concentration (15-125 ppm), decolorization ability of the bacteria divided in two parts: examinations with concentration  of less than 40 ppm and more than 40 ppm. The most effective factor was pH and increasing pH in a range of 6-10 caused a remarkable increase in decolorization. Temperature of 35-40˚C did not too have much effect on the decolorization. Comparison of arising spectrums from HPLC and UV-Vis before and after decolorization and observing the totomery ph enomenon in arising spectrums from IR confirmsthe considerable decolorization. Because of halopHilic and halotolerant bacteria, the amount of %5 (W/V) Sodium Chloride was added during all the tests.
 

Keywords


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