Isolation of Starch Degrading Bacteria present in Fath Well Drilling Fluid No. 32 Located in the South Azadegan Oil Field

Document Type : Research Paper

Abstract

Biodegradation of starch used in drilling fluid by many microorganisms, result in reduction of starch concentration in the fluid which leads to fluid efficiency downfall. Present study has been done with the aim of identification of major starch degrading bacteria in the drilling fluid and manner of controling the growth and activity of these organisms. Bacteria peresent in back up drilling fluid,were  isolated and amylase  activity was studied by starch hydrolysis test. Strain with most starch hydrolysis ability in all three temperatures (37, 45 and 55°C) were selected as superior strain and was identified by 16S rRNA. Also growth curve of superior strain was plotted. 54 bacterial strains include  26 Gram-positive bacillus, 22 Gram-negative bacillus and 6 Gram-positive cocci was isolated. 18 strains from 26 Gram-positive bacillus was sporated. Three strains in all three temperatures(37, 45 and 55°C) had growth and significant amylase activity. Bacteria with the ability to create maximum clear halo zone of amylase were selected as superior strain. The results of 16S rRNA analysis showed 99.8% similarity of superior strain to Bacillus licheniformis. Sporated Gram-positive bacillus belongs to Bacillus jenus like Bacillus licheniformis isolataed in this study was the most active degrading bacteria in well special conditions. Identification of degradation factors with the aim of selection of a suitable biocide in order to prevent biodegredeation is recommended.
 

Keywords


[1]. Leja k. and Lewandowicz G., “Polymer Biodegradation and Biodegradable Polymers-a Review,” Polish J. of Environ. Stud. Vol. 19, No. 2, pp. 255-256, 2010.##
[2]. Turkiewicz A., “The rol of microorganisms in the oil and gas industry,” Tom. 13, ISSN, 1506-218X, pp. 227-240, 2011.##
[3]. Davis J. B. and Updegraff D. M., “Microbiology in the Petroleum Industry,” Bactoriology Reviews, Vol. 18, pp. 229, 1954.##
[4]. Paulsen J. E., Omland TH., Igeltjorn H., Aas N. and Solvang, S. A., “Drill cuttings disposal, balancing zero discharge and use of best available technique,” SPE/IADC 85296, SPE/IADC Middle East Drilling Technology Conference & Exhibition, Abu Dhabi, UAE, pp. 1-11, 2003.##
[5]. Smith J. P., “Application of discharge modeling in environmental assessment of deepwater drilling discharge,” Presentation to meeting of the Petroleum Environmental Research Forum (PERF), Fall, Exxon Mobil Upstream Resaerch Co., Houston TX, 2003.##
[6]. Turkiewicz A., Brzeszcz J. and Kapusta P., “The application of biocides in the oil and gas industry, Oil & Gas Institue, pp. 103-112, Krakow, 2013.##
[7]. Jerry M. Neff, “Composition,Enviromental Fates, and Biological Effect of Water Based Drilling Muds and Cutting Discharged to the Marine Enviroment: A Synthesis and Annotated Bibliography,” Battelle, pp. 5, 11-13, 82, 2005.##
[8]. Nnubia C. and Okpokwasili G., “The microbiology of drill mud cutting from a new-offshore oilfield in nigeria,” Invironmental Polloution, 82, pp. 153-156, 1992.##
[10]. Cazorla F., Romero D., Perez-Gorcia A., Lagtenberg B., De Vicente A. and Bloemberg G, “Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity,” Gor. Applied Microbiology ISSN, PP. 1364-1370, 2007.##
[9]. Nweke C. O. and Okpokwasili G. C., “Drilling fluid base oil biodegradation potentional of a soil staphylococcus species,” African Journal Biotechnology, Vol. 2(9), pp. 293-295, 2003.##
[11]. Roland M., “HANDBOOK OF Microbiological Media,” 4th, CRC Press U. S. A., 2004.##
[12]. Wongsa P., “Isolation and characterization of novel strain of pseudomonas aeroginosa and serratia marcescense possessing high efficiency to degrade gasoline, kerosene, disel oil, and lubricating oil,” Current Microbiology, Vol. 49, pp. 415- 422, 2004.##
[13]. Joo M., Hur S., Han Y., and Kim J., “Isolation, identification and characterization of Bacillus strains from the traditional korean soybean-fermented food, chungkookjang,” Journal of Applied Biology and Chemistry, 50 (4), pp. 202-210, 2007.##
[14]. Kapusta P. and Turkiewicz A., “Investigations on microbiological degradation of polymers applied in drilling fluids technology,” Tom 11, pp. 1213-1224, 2009.##
[15]. Mavroudis D., “Downhole environmental risks associated with drilling and well completion practices in the cooper/eromanga basins,” Report Book 2001, Department of Primary Industries and Resources South Australia, p. 11, 2001.##