Separation of BiosurfactantGenerating Bacteria from Gachsaran Oil Reservoirs and Optimization of their Growth Conditions

Document Type : Research Paper

Authors

1 Islamic Azad University, Tehran Science and Research,

2 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University

3 Department of Petroleum, Firoozabad Branch, Islamic Azad University

4 Institute of Biotechnology, Shiraz University

Abstract

Recent evidence on the efficiency of microorganisms and their metabolic products in the oil extraction rate, which is also called microbial enhanced oil recovery, has been reported. In this work, the oil samples produced from Gachsaran oil reservoirs were evaluated in order to isolate and identify bacteria by using public and private media and to consider the ability to produce bio surfactant. In order to determine the best conditions for cell growth, carbon sources (whey, soy, starch, molasses, peas, and water) were added to the medium and bacterial growth curves were plotted over a period of 7 days for each of the bacteria. Best carbon sources for bacterial growth were whey, starch, and soy. The identification of bacteria was also done by molecular and biochemical methods. On the whole, according to the results of biochemical and 16sr RNA tests, these bacteria were classified in the Bacillus genus. Moreover, the sample No. 5 from bacteria Bacillus Licheniformis with ID 1455550 and the sample No. 7 from bacteria Bacillus Subtillis, with ID 1455557 were registered in the NCBI GenBank of America.

Keywords

References

مراجع
[1] Trondheim O.S. An Experimental Study of Viscous Surfactant Flooding for Enhanced Oil Recover, Master Thesis, 2005.
[2] Okpokwasili G.C., and Ibiene A.A. “Enhancement of recovery of residual oil using a biosurfactant slug’’, African Journal of Biotechnology,Vol. 5, No. 5, pp. 453-456, 2006.
[3] Henson M.A. “Exploiting cellular biology to manufacture high-value products’’, IEEE Control Systems Magazine., pp. 54-62, 2006.
[4] Myers D., Surfactant Science and Technology, Hoboken, New Jersey: John Wiley and Sons, Inc., 2006.
[5] Singh A., Van Hamme J.D., and Ward O.P. “Surfactants in microbiology and biotechnology:Part 2.”, Application aspects. Biotechnology Advances, Vol. 25 No. 1, pp. 99-121., 2007.
[6] Namani M. and Haghighi M., “Investigation of the Water Coning Phenomenon in Iranian Carbonate Fractured Reservoirs”, SPE 108254, Presented at the International Oil Conference and Exhibition, Mexico, June 2007.
[7] Amy P.S and Haldeman D.L., (eds), CRV Press, 356, 1997.
[8] Neidleman S.L., and Geigert J., “Biotechnology and oleochemicals: changing patterns,’’ J. Am. Oil Chem. Soc. Vol. 61, pp. 290-297, 1984.
[9] Morikawa M., daido H., Takao T., murata S., Shimonishi Y., and Imanaka T., “A new lipopeptide biosurfactant producedby Arthrobacter sp. strain MIS38,’’ J. Bacteriol., pp. 6459- 6466, 1993.
[10] Thaniyavarn J., Chongchin A., Wanitsuksombut N., Thaniyavarn S., Pinphanichakarn P. and Leepipatpiboon N. “Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source,’’ J. Gen. Appl. Microbiol., Vol. 52, pp. 215-222, 2006.
[11] Duvnjak Z., Cooper D.G. and Kosaric N. “Production of surfactant by Arthrobacter paraffineus ATCC 19558,’’ Biotechnol. Bioeng. Vol. 24, pp.165-175, 1982.
[12] Kosaric, N. “Biosurfactants in industry,’’ Pure and Appl. Chern.,Vol. 64, No. 11, pp. 1731-1737, 1992.
[13] Chandler D.P., Brockman F.J.,Bailey T.J. and Fredrickson J.K., Microb Ecol.;36, :37-50, 1998.
[14] Pedersen K., Arlinger J., Ekendahl S. and Hallbeck L., FEMS Microbiol Ecol.;19, :249-262, 1996.
Journal of Petroleum Research
Volume 22, Issue 69 - Serial Number 69
May and June 2012
Pages 33-41

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