عنوان مقاله [English]
Recent evidence on the efficiency of microorganisms and their metabolic products in the oil extraction rate, which is also called microbial enhanced oil recovery, has been reported. In this work, the oil samples produced from Gachsaran oil reservoirs were evaluated in order to isolate and identify bacteria by using public and private media and to consider the ability to produce bio surfactant. In order to determine the best conditions for cell growth, carbon sources (whey, soy, starch, molasses, peas, and water) were added to the medium and bacterial growth curves were plotted over a period of 7 days for each of the bacteria. Best carbon sources for bacterial growth were whey, starch, and soy. The identification of bacteria was also done by molecular and biochemical methods. On the whole, according to the results of biochemical and 16sr RNA tests, these bacteria were classified in the Bacillus genus. Moreover, the sample No. 5 from bacteria Bacillus Licheniformis with ID 1455550 and the sample No. 7 from bacteria Bacillus Subtillis, with ID 1455557 were registered in the NCBI GenBank of America.