بررسی پارامترهای مؤثر بر ارتقا برش نفت کوره با استفاده از میکروارگانیسم‌های سودوموناس آیروژینوس و رودوکوکوس اریتروپلیس جدا شده از لجن نفتی

نوع مقاله : مقاله پژوهشی

نویسندگان

آزمایشگاه تحقیقاتی فرآیندهای بیوتکنولوژی، دانشکده مهندسی شیمی و نفت، دانشگاه رازی، کرمانشاه، ایران

چکیده

در این مطالعه قابلیت باکتری‌های مفید جدا شده از لجن مخازن نفت پالایشگاه کرمانشاه و تأثیر شرایط محیط کشت جهت اصلاح نفت کوره مورد بررسی قرار گرفت. جهت جداسازی و خالص‌سازی میکروارگانیسم‌ها از محیط‌های کشت مایع و جامد استفاده گردید و طی مراحل مختلف میزان جمعیت میکروبی افزایش پیدا نمود. باکتری‌های فوق شامل میکروارگانیسم‌های سودوموناس آیروژینوس و رودوکوکوس اریتروپلیس هستند. در انجام آزمایش‌ها، متغیرهای انتخابی شامل پارامترهای pH، دما، زمان، غلظت نفت کوره و غلظت میکروارگانیسم‌ها بوده و هریک از متغیر‌ها در سطوح مختلف براساس شرایط استفاده گردید. همچنین، تأثیر مواد فعال‌سطحی بر اصلاح نفت کوره مورد بررسی قرار گرفت. براساس نتایج به‌دست آمده بهترین شرایط حذف ترکیبات گوگردی و تبدیل به برش‌های سبک‌تر در 5/6 =pH، دمای C° 40، غلظت 4% حجمی نفت کوره، میزان 2% حجمی محیط کشت حاوی دو میکروارگانیسم و زمان 3 روز، همراه با اضافه نمودن مواد فعال‌سطحی شیمیایی حاصل گردیده است , در پایان آنالیز، ترکیبات نمونه حاصل در بهترین شرایط آزمایش با روش SARA مشخص گردید. میزان گوگرد کل و دانسیته در نمونه اولیه به‌ترتیب ppm 25400 و kg/L 9428/0 و در نمونه نهایی برابر با ppm 9400 و kg/L 9096/0 است، که در مقایسه با نتایج نمونه اصلی میزان تبدیل نفت کوره به نفت گاز برابر با 3/29% و کاهش گوگرد کل بیش از 60% را نشان می‌دهد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of the Effective Parameters on the Upgrading of Fuel Oil by Pseudomonas Aeruginus and Rhodococcus Erythropolis Microorganisms Isolated from Oil Sludge

نویسندگان [English]

  • Nematolah Najafi
  • Farshad Rahimpour
Biotechnology Research Laboratory, Chemical Engineering Department, Faculty of Petroleum and Chemical Engineering, Razi University, Kermanshah, Iran
چکیده [English]

In this study, the capability of local bacteria, isolated from oily sludge in Kermanshah refinery oil tanks, and cultivation environment conditions effects on fuel oil upgrading were investigated. In order to isolate and purify microorganisms, liquid and solid culture media were used, and the amount of microbial population increased during different stages. The isolated bacteria include the Pseudomonas aeruginosa and Rhodococus erythropolis. In these experiments, the selected variables including pH, temperature, time parameters, fuel oil concentration and microorganism concentration were used at different levels based on the conditions. Density and total sulfur reduction were used as responses parameters. Also, the effect of chemical surfactant on the responses was studied. Based on the achieved results, the best conditions for sulfur removal and converting heavy cut to ligher one, were obtained at pH = 6.5, temperature of 40 °C, fuel oil concentration 0f 4 % (V/V) microorganisms’ concentration of 2 % (V/V) and 3 days, together with the addition of chemical surfactant. The SARA analysis results shown that total sulfur and density in untreated fuel oil were 25400 ppm and 0.9428 kg/L, respectively, and in optimal treated one, they were 9400 ppm and 0.9096 kg/L, which it demonstrated that the conversion rate of fuel oil to gas oil was equal to 29.3% and reduction of total sulfur by more than 60%.
 

کلیدواژه‌ها [English]

  • Fuel oil
  • Pseudomonas aeruginus
  • Rhodococcus erythropolis
  • Heavy oil upgrade
  • SARA
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