تأثیر گلوکز و زایلوز روی رشد و تولید روغن تک یاخته توسط سویه‌های مخمری منتخب

نوع مقاله: مقاله پژوهشی

نویسندگان

دانشکده علوم، دانشگاه اصفهان، گروه زیست شناسی، بخش میکروبیولوژی

چکیده

استفاده از قندهای حاصل از هیدرولیز ترکیبات لیگنوسلولزی راهکار مناسبی برای تولید بیودیزل مخمری است. در این تحقیق برای شناسایی سویه‌های مخمری مولد چربی از رنگ‌آمیزی با سودان سیاه و رنگ فلورسنت نایل رد استفاده شد. رشد و تولید لیپید با استفاده از گلوکز، زایلوز و نیز مخلوط گلوکز و زایلوز به عنوان منبع کربنی در 11 سویه مخمری منتخب بومی و 2 سویه مخمری از کلکسیون میکروبی DSMZ مورد بررسی قرار گرفت. نتایج نشان داد سویه‌های بومی توانایی تولید مقادیر بالایی از لیپید را دارا می‌باشد، در نتیجه می‌توان از آن برای تولید این محصول استفاده کرد. در این تحقیق براساس نوع سویه و منبع کربن، میزان تولید لیپید بین gr/lit 21/0 تا 95/6، میزان زی توده خشک سلولی بینg/lit 73/0 تا 9/10، محتوای لیپیدی بین 4/26% تا 4/81% و کارآیی تولید لیپید بین 41/6% تا 95/21% متغیر بود. بیشترین محتوای لیپیدی (4/81 %) و کارآیی تولید لیپید (95/21%) توسط سویه UIMC65 به دست آمد. بیشترین میزان تولید لیپید (gr/lit 95/6) مربوط به سویه‌های UIMC35 (در حضور مخلوط گلوکز و زایلوز) و UIMC74 (درحضور گلوکز) بود. از تکنیک‌های TLC و FT-IRspectroscopy به منظور ارزیابی کیفی روغن تک یاخته تولیدی استفاده گردید.
 

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Glucose and Xylose on Growth and Single Cell Oil Production by Selected Oleaginous Yeast Strains

نویسندگان [English]

  • Hossein Ghanavati
  • Iraj Nahvi
چکیده [English]

Using the sugars from lignocelluloses hydrolysate is a suitable strategy for biodiesel production by yeasts. In this work, Sudan black dye and Nile red florescent dye were used for staining and selecting the oleaginous yeast strains. Growth and single cell oil production in 11 selected native yeasts and 2 yeasts from microbial collection of DSMZ were investigated by using glucose, xylose, and a mixture of both as carbon sources. The results showed that native yeasts had a good sufficient potential for lipid production in the industrial field. According to the type of strain and carbon source, lipid production, dry cell weight, lipid content, and lipid production efficiency were achieved to be 0.21-6.95 g/l, 0.73-10.9 g/l, 26.4-81.4%, and 6.41-21.95% respectively. Among the investigated yeast strains, the highest lipid content (81.4%) and lipid production efficiency (21.95%) were related to strain UIMC65. The highest lipid production (6.95 g/l) was acquired in strain UIMC35 (a mixture of glucose and xylose as the carbon source) and strain UIMC74 (glucose as the carbon source). TLC and FT-IR spectroscopy techniques were used for the quality analysis of produced single cell oil by yeast strains.
 

کلیدواژه‌ها [English]

  • Oleaginous Yeast Strains
  • Single Cell Oil (SCO)
  • Biodiesel
  • Glucose
  • Xylose
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