بررسی توانایی سیانوباکتری Fischerellaambigua ISC67 در تجزیه زیستی نفت خام

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشگاه ایلام

2 دانشگاه مازندران

3 دانشگاه شهید بهشتی تهران، پژوهشکده علوم پایه کاربردی جهاد دانشگاهی

چکیده

هدف اصلی از این مطالعه بررسی توانایی سیانوباکتری ‏ Fischerella ambigua ISC67‎‏ در تجزیه زیستی نفت خام و اثر نفت خام بر پاسخ‌های فیزیولوژیک این سیانوباکتر می‌باشد. در این مطالعه تجربی، سیانوباکتری Fischerella ambiguaISC67 از کلکسیون ریز جلبک‌ها تهیه شد‏. نرخ رشد سیانوباکتری در آزمایه 1% نفت خام در طول موج nm 750 و مقدار کلروفیل در آزمایه های 5/0، 1، 2 و 4% نفت خام در طول موج nm 665 با استفاده از دستگاه اسپکتوفتومتر اندازه‌‌گیری شد. برای تعیین وزن خشک، با استفاده از کاغذ صافی‌های وزن شده، سیانوباکتر از محیط کشت جدا گردید و پس از خشک شدن وزن آنها اندازه‌گیری شد. میزان تجزیه نفت خام به روش آنالیز کروماتوگرافی گازی محاسبه گردید. نتایج نشان داد که رشد این سیانوباکتری در حضور نفت خام تقریباً مشابه با نمونه شاهد افزایش می‌یابد، به طوری که این افزایش نرخ رشد تقریباً مشابه و گاهی کمتر از نمونه شاهد می‌باشد. همچنین با افزایش غلظت نفت خام، میزان وزن خشک سیانوباکتری در آزمایه های 5/0 و 2% نسبت به نمونه شاهد افزایش و میزان کلروفیل در آزمایه‌های مختلف نفت خام کاهش می‌یابد. میانگین تجزیه زیستی هیدرکربن‌های نفتی در آزمایه های روز 14 نسبت به نمونه شاهد 32/42% و در روز 28 به مقدار 21/54% بود. در این مطالعه مشخص شد که سیانوباکتری Fischerella ambigua دارای توانایی بالایی در تجزیه زیستی نفت خام است. لذا نتایج حاصل، بیان‌گر قابلیت کاربرد این سیانوباکتری به‌عنوان شاخصی جهت رفع آلودگی‌های نفتی در مناطق آلوده می‌باشد.‏
 

کلیدواژه‌ها


عنوان مقاله [English]

The Potential of Cyanobacterium Fischerella Ambigua ISC67 ‎in Biodegradation of Crude Oil

نویسنده [English]

  • Moein Safari 1
چکیده [English]

The main objective of this study is to investigate the potential of cyanobacterium Fischerella ambigua ISC67‎ in the biodegradation of crude oil and evaluation of oil effect on physiological responses. In this experimental study, the cyanobacterium Fischerella ambigua ‎ ISC67 was obtained from the algal ‎culture collection. The growth rate of cyanobacteria in 1% treatment and control sample of crude oil measured at ‎‎750 nm and chlorophyll content in 0.5, 1, 2, and 4% treatments of crude oil measured at h nm ‎‎665nm wavelength using a spectrophotometer. For measuring of dry weight, firstly‎ using weighed ‎filter paper cyanobacteria were isolated from the culture medium and their weights were measured ‎after drying. ‎The rate of biodegradation of crude oil analyzed by gas chromatography. The results showed that the growth of this ‎cyanobacterium in the presence  of crude oil is almost same as of control sample.‎ So that these growth rates are nearly equal and sometimes lower than the control sample. Also, with increasing concentrations of crude oil, dry weight of this cyanobacterium in 0.5% and 2% treatments increased compared to the control sample and it chlorophyll rate in the various treatments of crude is reduced The average of petroleum hydrocarbon biodegradation in 14 days of treatment compared to the control sample was at a rate of 42.32 % and and 28 days was 54.21% respectively. In this study it was found that cyanobacterium Fischerella ambigua ISC67‎ has great potential in biodegradation of crude oil. Therefore, these results indicate the potential of this cyanobacterium for its use as an indicator to eliminate pollution in contaminated areas.
 

کلیدواژه‌ها [English]

  • Cyanobacteria
  • Biodegradation
  • Crude Oil
  • Chlorophyll
  • Gas Chromatography

 

[1]. Hassanshahian M., Emtiazi G., Kermanshahi R. and Cappello S., “Comparison of oil degrading microbial communities in sediments from the Persian Gulf and Caspian Sea, Soil and Sediment Contamination,” Vol. 19, No. 3, pp. 277–291, 2010.##

[2]. Baek K. H., Kim H. S., Oh H. M., Yoon B. D., Kim J. and Lee I. S., “Effects of crude oil, oil components, and bioremediation on plant growth,” Journal of Environmental Science and Health, Vol. 39 No. 9, pp. 2465–2472, 2004.##

[3]. Ma F., Shi Sh., Sun T. H., Li A., Zhou J. T. and Qu Y. Y., “Biotransformation of benzene and toluene to catechols by phenol hydroxylase from Arthrobacter sp. W1,”. Applied Microbiology and Biotechnology, Vol. 97, No. 11, pp. 5097-103, 2012.##

[4]. Kastner M., “Degradation of aromatic and polyaromatic compounds, Biotechnology,” Environmental Processes. Germany: Wiley Vch., 2000.##

[5]. Rashid-Ashmagh F., Rezaei-Kalantary R., Farzadkia M., Joneidy-Jafari A. and Nabizadeh R., “Survey of phenantherene biodegradation model in contaminated soils by acinetobacter SP.,” Iran. J. Health & Environ, 2 (3), pp. 196-203, 2009. (in Persian)##

[6]. Gennaro P. Di, Franzetti A., Bestetti G., Lasagni M, Pitea D. and Collina E., “Slurry phase bioremediation of PAHs in industrial landfill samples at laboratory scale,” Waste Management, 28 (8), pp. 1338-1345, 2007.##

[7]. Hassanshahian M., Emtiazi G. and Cappello S., “Isolation and characterization of crude-oil degrading bacteria from the persian gulf and the caspian sea,” Marine Pollution Bulletin, 64, pp. 7–12, 2012.##

[8]. El-Sheekh M. M., Hamouda R. A. and Nizam A. A., “Biodegradation of crude oil by Scenedesmus obliquus and Chlorella vulgaris growing under heterotrophic conditions,” International Biodeterioration and Biodegradation, 82, pp. 67-72, 2013.##

[9]. Chena B. and Ding J., “Biosorption and biodegradation of phenanthrene and pyrene in sterilized and unsterilized soil slurry systems stimulated by phanerochaete chrysosporium,” Journal of Hazardous Materials, 229, pp. 159– 169, 2012.##

[10]. Erdogan E., and Karaca A., “Bioremediation of curd oil polluted soils,” Asian Journal of Biotechnology, 3(3), pp. 206-213, 2011.##

[11]. Xu R., and Obbard J. P., “Effect of nutrient amendments on indigenous hydrocarbon biodegradation in oil contaminated beach sediments,” Journal of Environmental Quality, 32, pp. 1234-1243, 2003.##

[12]. Hassanshahian M., Hassanshahian O. and Emtiazi G., “Optimization of biodegradation of crude oil by Acinetobacter calcoacticus BS and Pseudomonas aeroginosa AS bacteria isolated from Persian Gulf,” Petroleum Research, 20 (63), pp. 72-82, 2010.##

[13]. Abed R. M. M., Dobretsov S. and Sudesh K., “Applications of cyanobacteria in biotechnology,” Journal of Applied Microbiology, 106, pp. 1-12, 2009.##

[14]. Patel A., Pawar R., Mishra S. and Tewari A., “Exploitation of marine cyanobacteria for removal of color from distillery effluent,” Indian Journal of Environmental Protection, 21 (12), pp. 1118–1121, 2001.##

[15]. Chaillan F., Gugger M., Saliot A., Coute A. and Oudot J., “Role of cyanobacteria in the biodegradation of crude oil by a tropical cyanobacterial mat,” Chemosphere, 62, pp. 1574-1582, 2006.##

[16]. Ellis B. E., “Degradation of phenolic compounds by freshwater algea,” Plant Science Letters, 8, pp. 213-216, 1977.##

[17]. Kuritz T. and Wolk C. P., “Use of filamentous cyanobacteria for biodegradation of organic pollutants,” Applied and Enviromental Microbiology, 61 (1), pp. 234-238, 1995.##

[18]. Raghukumar C., Vipparty V., David J. J. and Chandramohan D., Degradation of crude oil by cyanobacteria, Applied Microbiology and Biotechnology, 57, pp. 433–436, 2001.##

[19]. Ibraheem I. B. M., “Biodegradation of hydrocarbons by cyanobacteria,” Journal of Phycology, 46, pp. 818–824, 2010.##

[20]. Andersen R. A., “Algal culturing techniques,” Elsevier Academic Press, pp. 16-18, 2005.##

[21]. Allen M. M. and Stanierr Y., “Growth and division of some unicellular blue-green algae,” Journal of General Microbiology, (51), pp. 199- 202, 1986.##

[22]. Soltani N., Khavari-Nejad R. A., Yazdi M.T., Shokravi S. and Fernández-Valiente E., “Screening of soil cyanobacteria for antifungal and antibacterial activity,” Pharm Biol. 43, pp. 455-459, 2005.##

[23]. Marker A. F. H., “The use of acetone and methanol in the estimated of chlorophyll in the presence of phaeophytin,” Freshwater Boil, 2, pp. 361-385, 1972.##

[24]. Leganes F., Sanches-Maeso E. and Fernandez-Valiente E., “Effect of indolacetic acid on growth and dinitrogen fixation in cyanobacteria,” Plant Cell Physiology, 28, pp. 529-533, 1987.##

[25]. Abed R. M. M., “Interaction between cyanobacteria and aerobic heterotrophic bacteria in the degradation of hydrocarbons,” International Biodeterioration & Biodegradation, 64, pp. 58-64, 2010.##

[26]. Ali-Gamila H., Ibrahim M. B. M and Abd R. M. and El-Ghafar H. H., “The role of cyanobacteria isolated strains in the biodegradation of crude oil,”International Journal of Environmental Studies,” 60, pp. 435-444, 2003.##

[27]. Gaur J. P., and Kumar H. D., “Growth response of four micro-algae to three crude oils and furnace oil,” Environmental Pollution, 25, pp. 77-85, 1981.##

[28]. Kumar S., Habib K. and Fatma T., “Endosulfan induced biochemical changes in nitrogen fixing cyanobacteria,” Science of Total Environment, 403, pp. 130-138, 2008.##

[29]. Ouzounidou G., “Effect of copper on germination and seedling growth of Minuatia, Silene, Alyssum and Thlaspi,” Biology of Plants, 37, pp. 411–416, 1995.##

[30]. Sundaram S., and Soumya K. K., “Study of Physiological Alterations in Cyanobacterium under Organic Stress,” American Journal of Plant Physiology, 6(1), pp. 1-16, 2011.##

[31]. El-Sheekh M. M. and Hamouda R. A., “Biodegradation of crude oil by some cyanobacteria under heterotrophic conditions,” Desalination and Water Treatment, 1, pp. 1-7, 2013.##

[32]. Pimda W., and Bunnag S., “Biodegradation of used motor oil by Nostoc piscinale TISTR 8401,” African Journal of Microbiology Research, 6 (10), pp. 2367-2372, 2012.##

[33]. Al-Hasan R. H., Khanafar M., Eliyas M. and Radwan S. S., “Hydrocarbon accumulation by pcocyanobacteria from Persian Gulf,” Journal of Applied Microbiology, 91, pp. 533-540, 2001.##

[34]. Abed R. M. M. and Koster J., “The direct role of aerobic heterotrophic bacteria associated with cyanobacteria in the degradation of oil compounds,” International Biodeterioration and Biodegradation, 55, pp. 29-37, 2005.##

[35]. Hoehler T. M., Bebout B. M. and Des-Marais D. J., “The role of microbial mats in the production of reduced gases on the early Earth,” Nature, 412 (6844), pp. 324–327, 2001.##

[36]. Abed R. M. M., Safi N. M. D., Koster J., Beer D., El-Nahhal Y., Rullkotter J. and Garcia Pichel F., “Microbial diversity of a heavily polluted microbial mat and its community changes following degradation of petroleum compounds,” Applied and Environmental Microbiology, 68, pp. 1674–1683, 2002.##