بررسی تجربی و مدل‌سازی ترمودینامیکی فرآیند استخراج لیپید از ریزجلبک مرطوب

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشکده مهندسی شیمی و نفت، دانشگاه صنعتی شریف، تهران، ایران

2 مرکز تحقیقات مهندسی بیوشیمی و کنترل محیط زیست، دانشگاه صنعتی شریف، تهران، ایران

3 دانشکده مهندسی شیمی و نفت، دانشگاه صنعتی شریف، تهران، ایران/ مرکز تحقیقات مهندسی بیوشیمی و کنترل محیط زیست، دانشگاه صنعتی شریف، تهران، ایران

چکیده

در این مقاله، نسبت‌های مختلف متانول به‏عنوان حلال قطبی و هگزان به‏عنوان حلال ناقطبی برای استخراج لیپید از ریزجلبک خشک و مرطوب با رطوبت‌های 70 و 85 درصد استفاده شدند. مقدار لیپید استخراجی تا نسبت هم‌حجم مخلوط هگزان و متانول روند صعودی دارد که با افزایش بیش‏تر متانول این روند کاهش می‌یابد. یک کاهش عمده‌ لیپیدهای خام استخراجی با افزایش رطوبت در نسبت‌های پایین متانول به هگزان مشاهده شد. بیش‏ترین مقدار اسیدهای چرب استخراجی با استفاده از مخلوط هم‌حجم هگزان:متانول به دست آمد(53/0±30/2 درصد). با افزایش رطوبت بازیابی اسید چرب کاهش یافت، ولی پروفایل اسیدهای چرب برای مخلوط حلال متفاوت تقریباً مشابه با ریزجلبک خشک بود. بیش‏ترین بازیابی اسید چرب با اعمال مخلوط هم‌حجم هگزان:متانول به‏عنوان حلال به دست آمد که درصد آن برای رطوبت 70 و 85 درصد زیست‌توده به‏ترتیب 33/0±2/2 و 16/0±95/0 است. هم‏چنین اثر دما بر عمل‏کرد استخراج نیز بررسی شد که نتایج نشان دادند بازیابی لیپید خام و اسید چرب با افزایش دما بهبود می‌یابد(به‏ترتیب 17 و 36 درصد برای دمای  65 درجه سانتی‌گراد در مقایسه با 25 درجه سانتی‌گراد). مدل ترمودینامیکی UNIQUAC برای تحقیق درباره‌ تاثیر مقدار رطوبت و نسبت حلال بر فرآیند استخراج لیپید به کار گرفته شد. پارامترهای برهم‌کنش انرژی با به‏کارگیری داده‌های تجربی به دست آمدند. نتایج تخمینی با 65/8 درصد>AAD درصد دقت مدل UNIQUAC را اثبات کردند.
 

کلیدواژه‌ها


عنوان مقاله [English]

Experimental Study and Thermodynamic Modeling of the Lipid Extraction Process from Wet Microalgae

نویسندگان [English]

  • Mohammad Malekzadeh 1
  • Hamed Abedini-Najafabadi 1 2
  • Manouchehr Vossoughi 3
  • Davood Rashtchian 1
1 Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran
2 Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran| Institute for Biotechnology and Environment (IBE), Sharif University of Technology, Tehran, Iran
3 Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran/ Institute for Biotechnology and Environment (IBE), Sharif University of Technology, Tehran, Iran
چکیده [English]

In this study, different ratios of methanol and hexane were used for lipid extraction from lyophilized microalgae and wet biomasses with 70 and 85 % moisture content. The amount of extracted crude lipid showed an increasing trend up to 1:1 volumetric ratio of methanol and hexane. Nevertheless, the increasing trend was diminished by further increase of methanol. A significant decrease of extracted crude lipids was observed with increase of moisture in low ratios of methanol. The highest amount of extracted fatty acids was achieved when same ratio of methanol and hexane were used, and amount of it was 2.30±0.53. The fatty acid recovery was decreased by increasing the moisture content. However, the profile of extracted fatty acids for different solvent mixture was nearly the same as dried microalgae. The best performance of extraction belongs to 1:1 hexane/methanol ratio which was 2.2±0.33 and 0.95±0.16 for 70 and 85% moisture content of biomass, respectively. The effect of temperature on the performance of extraction was also investigated, and the results showed that the recovery of both crude lipids and fatty acids was improved by increase of temperature, in which the crude lipid and fatty acid recovery was increased by about 17 and 36% at 65 oC comparing to the 25 oC. UNIQUAC thermodynamic model was employed to investigate the influences of moisture content and solvent ratio on lipid extraction process. Energy interaction parameters of the model were obtained by utilization of experimental data. Estimated results with AAD (%) inferior to 8.65 proved accuracy of it.
 

کلیدواژه‌ها [English]

  • Microalgae
  • Chlorella Vulgaris
  • Lipid
  • Extraction
  • Thermodynamic Modeling

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