نوع مقاله: مقاله پژوهشی
نویسندگان
1 گروه بیوتکنولوژی، دانشکده مهندسی شیمی، دانشگاه صنعتی نوشیروانی بابل، ایران
2 گروه فیزیک، دانشکده علوم پایه، دانشگاه صنعتی نوشیروانی بابل، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Nowadays, biosurfactants are widely used in various industries due to their wide range of applications. In this paper, the production of rhamnolipid biosurfactant from Pseudomonas aeruginosa PTCC 1340 as one of the most important biosurfactants was investigated using a sunflower oil carbon source. For this purpose, soil samples were contaminated by crude oil with API 33 artificially. In this study, the production of biosurfactant in the removal of crude oil was investigated. Moreover, the results of this study showed that the maximum amount of rhamnolipid production was 4.3 g/L. Rhamnolipid structure was examined and verified by TLC and FTIR analyzes. The study of the properties of the produced rhamnolipid showed that these substances had a high potential in reduction of surface tension, as the produced rhamnolipid reduced the surface tension of water from 69 mN/m to 26.8 mN/m. It also showed that a considerable stability in mixing with NaCl salt up to a concentration of 15%. Finally, the results showed that Rhamnolipid supernatant eliminated 75.17% of the crude oil in the soil, while the highest removal efficiency of the crude oil from the soil was 76.92% by SDS surfactant. According to the successfull results, it can be suggested that the produced rhamnolipid biosurfactant can be used as an alternative to chemical surfactants in various industries.
کلیدواژهها [English]
[1]. امانی ح.، "تولید بیوسورفکتنت های رامنولیپید به منظور کاربرد در فرآیند ازدیاد برداشت نفت ،" نشریه شیمی و مهندسی شیمی ایران، دوره 32، شماره 1، صفحات 83-73، 1392.
[9]. Rosen M. J. and Kunjappu J. T., “Surfactant and interfacial phanomena”, 4th ed., A John Wiley and Sons Inc., 2012. ##
[10]. Ceschia E., Switchable surfactants for soil remediation, MSc. Thesis. 2011. ##
[11]. Chu W. and Chan K. H., “The mechanism of the surfactant-aided soil washing system for hydrophobic and partial hydrophobic organics,” Science of The Total Environment, Vol. 307, No. 1, pp. 83-92, 2013. ##
[12]. Uzoigwe C., J. Ennis C., K. S. M. and Rahman P., “Production of Biosurfactants using Eco-friendly Microorganisms”, Environmental Sustainability, 2015. ##
[13]. Helmi W., Sarinah B., Murni H., Ahmad Firdaus B. L., Arbakaria B. A. and Oi-Ming L., “Production of Rhamnolipids by Locally Isolated Pseudomonas aeruginosa using Sunflower Oil as Carbon Source”, Vol. 5, No. 1, pp. 1-6, 2017. ##
[14]. Lan G., Fan Q., Liu Y., Chen CH., Li G., Liu Y. and Yin X., “Rhamnolipid production from waste cooking oil using pseudomonas SWP-4”, Biochemical Engineering Journal, Vol. 101, pp. 44-54, 2015. ##
[15]. Zulfigar A. R., Muhammad S.Kh., Zafar M. Kh. and Asma R., “Production of biosurfactant using different hydrocarbons by pseudomonas aeruginosa EBN-8 mutant”, Journal of Biosciences Zeitschrift für Naturforschung C, Vol. 61, No. 2, 2006. ##
[16]. Urum K., Pekdemir T. and Gopur M., “Optimum conditions for washing of crude oil-contaminated soil with biosurfactant solutions”, Vol. 81, No. 3, pp. 203-209, 2003. ##